Description Application This kit is for the quantitative, enzymatic determination of fructose in food and other materials.Fructose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Fructose 6-phosphate is converted to glucose 6-phosphate by phosphoglucose isomerase (PGI). Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in the reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to fructose concentration.
Properties usage sufficient for 20 assays storage temp. 2-8°C
References reference Beuter, H.O. Meth. Enzym. Anal. 3rd ed., 6, 321, (1985) Official Methods of Analysis of the AOAC 16th ed., Arlington, VA , (1995), 28.1.17
